Western blot was performed using Anti-SHP-1 Monoclonal Antibody (PTY6)(Product # MA1-21484) and a 63 kDa band corresponding to SHP-1 was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), HEL 92.1.7 (Lane 2), K-562 (Lane 3), U-2 OS (Lane 4), Mouse Spleen (Lane 5) and Mouse Skeletal Muscle (Lane 6), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). K-562 and U-2 OS cell lines and Skeletal Muscle tissue, are low SHP-1 expressing models, as reported.